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cd43 (ly-48) microbeads, mouse  (Miltenyi Biotec)


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    Miltenyi Biotec cd43 (ly-48) microbeads, mouse
    Cd43 (Ly 48) Microbeads, Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd43+ly+48+microbeads/CD43+(Ly-48)+MicroBeads%2C+mouse/custom%40130-049-801%4041665602
    Average 95 stars, based on 134 article reviews
    cd43 (ly-48) microbeads, mouse - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Relapse-founding cancer persister cells in follicular lymphoma
    Article Snippet: .. As previously described , splenic cells from donor mice were incubated with CD43 (Ly-48) MicroBeads (Miltenyi Biotec; 130-049-801) and enriched for B-cells by negative selection using MACS LS columns (Miltenyi Biotec). ..

    Selection:

    Article Title: Relapse-founding cancer persister cells in follicular lymphoma
    Article Snippet: .. As previously described , splenic cells from donor mice were incubated with CD43 (Ly-48) MicroBeads (Miltenyi Biotec; 130-049-801) and enriched for B-cells by negative selection using MACS LS columns (Miltenyi Biotec). ..

    Article Title: Chronic stress unleashes an intratumor phage-fibroblast-B cell circuit to promote tumor growth.
    Article Snippet: The lysis reaction was quenched by adding five volumes of B cell media (RPMI 1640 + l-glutamine (11875, GIBCO), 15% FBS (35-010-CV, Corning), and 1% penicillin–streptomycin (400-109, Gemini)). .. Following RBC lysis, B cells were isolated from total splenocytes by incubating with CD43 (Ly-48) MicroBeads (130-049-801, Miltenyi Biotec) in MACS buffer (500 mL PBS, 0.5% BSA, 0.5 M EDTA), followed by negative selection using LS Columns (130-042-401, Miltenyi Biotec). .. Naive B cells were seeded at 1 × 106 per mL of B cell media and cultured ex vivo with combinations of LPS (50 ng/mL; L4130, Sigma-Aldrich), IL-4 (12.5 ng/mL; 404-ML-010, R&D Systems), and anti-mouse CD40 (1 μg/mL; 12-0401-82, eBioscience) in presence or absence of 2μM corticosterone (27840, SigmaAldrich).

    Magnetic Cell Separation:

    Article Title: Relapse-founding cancer persister cells in follicular lymphoma
    Article Snippet: .. As previously described , splenic cells from donor mice were incubated with CD43 (Ly-48) MicroBeads (Miltenyi Biotec; 130-049-801) and enriched for B-cells by negative selection using MACS LS columns (Miltenyi Biotec). ..

    Article Title: PTEN-mTORC2 signaling module controls antibody isotype selection and antiviral humoral immunity
    Article Snippet: .. B cells were isolated by depleting CD43 + cells using CD43 (Ly-48) MicroBeads and MACS Separator (Miltenyi Biotec). .. GC B cells were purified using the Mouse Germinal Center B cell MicroBead Kit (Miltenyi Biotec) according to the manufacturer’s instructions.

    Article Title: Antibody-mediated feedback modulates interclonal competition in the germinal center.
    Article Snippet: J. Bilanovic was supported by a Boehringer Ingelheim Fonds PhD fellowship and by the National Cancer Institute of the National Institutes of Health under award number F99CA305580. .. REAGENT or RESOURCE SOURCE IDENTIFIER MACS Cell Separation Column LS Miltenyi Biotec Cat#130-042-401 CD43 (Ly-48) microbeads, mouse Miltenyi Biotec Cat#130-049-801 EZ-Link Sulfo-NHS-LC-LC-Biotin Thermo Fisher Scientific Cat#21338 Experimental models: Cell lines Chinese Hamster: CHO-DG44 cells D. Hacker (EPFL) Rajendra et al. 49 N/A NB-21.2D9 feeder cells G. Kelsoe (Duke University) Kuraoka et al. 38 N/A Experimental models: Organisms/strains Mouse: C57BL6/J The Jackson Laboratory JAX: 000664 Mouse: CD45.1 mice Ptprc K302E JAXBoy The Jackson Laboratory JAX: 033076 Mouse: CD23-Cre M. Busslinger (IMP Vienna) Kwon et al. 26 N/A Mouse: Prdm1 LSL-DTR This paper N/A Mouse: Prdm1 DTR This paper N/A Mouse: Aicda CreERT2 C-A Reynaud, J-C Weill (U. Paris-Descartes) Dogan et al. 28 N/A Mouse: Ig Sa This paper N/A Mouse: Ig Cb This paper N/A Mouse: Igk – This paper N/A Mouse: Bcl6 flox/flox A Dent (Indiana U.) .. Dent et al. 6 N/A Mouse: Cd79a Cre M. Reth (U. Freiburg) Hobeika et al. 36 N/A Mouse: Ig chIgY Rockefeller University DeWitt et al. 42 N/A Oligonucleotides CRISPR guide-RNA targeting the Prdm allele: TGAAAATCTTAAGGATCCAT This paper N/A CRISPR guide-RNA targeting the Igh allele #1: TCTCTACTTCCTCATAGCTC This paper N/A CRISPR guide-RNA targeting the Igh allele #2: GGAGCCGGCTGAGAGAAGTT This paper N/A CRISPR guide-RNA targeting the Igk allele #1: CTGTGGTGGACGTTCGGTGG This paper N/A CRISPR guide-RNA targeting the Igk allele #2: AAGACACAGGTTTTCATGTT This paper N/A CRISPR guide-RNA targeting the Igkc allele: GTTCAAGAAGCACACGACTG This paper N/A Recombinant DNA Plasmid: pVRC8400 A. McDermott (VRC/NIAID/NIH) N/A Software and algorithms Prism software Graphpad RRID: SCR_002798 FlowJo software FlowJo LLC RRID: SCR_008520 Excel Microsoft RRID: SCR_016137 Illustrator Adobe RRID: SCR_010279 Other 96-Well Half Area Microplates Greiner Bio-One Cat#675061 Prepared Microtubes, Z-Gel Sarstedt Cat#41.1378.005 OptiMEM Thermo Fisher Scientific Cat#31985070 e2 Immunity 59, 1–12.e1–e5, March 10, 2026

    Article Title: Chronic stress unleashes an intratumor phage-fibroblast-B cell circuit to promote tumor growth.
    Article Snippet: The lysis reaction was quenched by adding five volumes of B cell media (RPMI 1640 + l-glutamine (11875, GIBCO), 15% FBS (35-010-CV, Corning), and 1% penicillin–streptomycin (400-109, Gemini)). .. Following RBC lysis, B cells were isolated from total splenocytes by incubating with CD43 (Ly-48) MicroBeads (130-049-801, Miltenyi Biotec) in MACS buffer (500 mL PBS, 0.5% BSA, 0.5 M EDTA), followed by negative selection using LS Columns (130-042-401, Miltenyi Biotec). .. Naive B cells were seeded at 1 × 106 per mL of B cell media and cultured ex vivo with combinations of LPS (50 ng/mL; L4130, Sigma-Aldrich), IL-4 (12.5 ng/mL; 404-ML-010, R&D Systems), and anti-mouse CD40 (1 μg/mL; 12-0401-82, eBioscience) in presence or absence of 2μM corticosterone (27840, SigmaAldrich).

    Isolation:

    Article Title: PTEN-mTORC2 signaling module controls antibody isotype selection and antiviral humoral immunity
    Article Snippet: .. B cells were isolated by depleting CD43 + cells using CD43 (Ly-48) MicroBeads and MACS Separator (Miltenyi Biotec). .. GC B cells were purified using the Mouse Germinal Center B cell MicroBead Kit (Miltenyi Biotec) according to the manufacturer’s instructions.

    Article Title: Chronic stress unleashes an intratumor phage-fibroblast-B cell circuit to promote tumor growth.
    Article Snippet: The lysis reaction was quenched by adding five volumes of B cell media (RPMI 1640 + l-glutamine (11875, GIBCO), 15% FBS (35-010-CV, Corning), and 1% penicillin–streptomycin (400-109, Gemini)). .. Following RBC lysis, B cells were isolated from total splenocytes by incubating with CD43 (Ly-48) MicroBeads (130-049-801, Miltenyi Biotec) in MACS buffer (500 mL PBS, 0.5% BSA, 0.5 M EDTA), followed by negative selection using LS Columns (130-042-401, Miltenyi Biotec). .. Naive B cells were seeded at 1 × 106 per mL of B cell media and cultured ex vivo with combinations of LPS (50 ng/mL; L4130, Sigma-Aldrich), IL-4 (12.5 ng/mL; 404-ML-010, R&D Systems), and anti-mouse CD40 (1 μg/mL; 12-0401-82, eBioscience) in presence or absence of 2μM corticosterone (27840, SigmaAldrich).

    other:

    Article Title: HnRNP A1 and A2B1 enforce Ezh2 mRNA splicing to promote germinal center B cell responses.
    Article Snippet: For CaspGLOW apoptosis assays (BioVision, 180-100; Thermo, 88-7003-42), apoptosis-associated caspase activity was detected using the FITC-conjugated active caspase inhibitor VAD-FMK.

    Article Title: In vivo CRISPR/Cas9 screens identify new regulators of B cell activation and plasma cell differentiation.
    Article Snippet: The following monoclonal antibodies (clone, fluorophore, catalog number, manufacturer) were used for flow cytometric analysis of cells: CD19 (1D3, BV786, 563333; BD Biosciences),

    Red Blood Cell Lysis:

    Article Title: Chronic stress unleashes an intratumor phage-fibroblast-B cell circuit to promote tumor growth.
    Article Snippet: The lysis reaction was quenched by adding five volumes of B cell media (RPMI 1640 + l-glutamine (11875, GIBCO), 15% FBS (35-010-CV, Corning), and 1% penicillin–streptomycin (400-109, Gemini)). .. Following RBC lysis, B cells were isolated from total splenocytes by incubating with CD43 (Ly-48) MicroBeads (130-049-801, Miltenyi Biotec) in MACS buffer (500 mL PBS, 0.5% BSA, 0.5 M EDTA), followed by negative selection using LS Columns (130-042-401, Miltenyi Biotec). .. Naive B cells were seeded at 1 × 106 per mL of B cell media and cultured ex vivo with combinations of LPS (50 ng/mL; L4130, Sigma-Aldrich), IL-4 (12.5 ng/mL; 404-ML-010, R&D Systems), and anti-mouse CD40 (1 μg/mL; 12-0401-82, eBioscience) in presence or absence of 2μM corticosterone (27840, SigmaAldrich).



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